Effects of ketamine on myocardial NF-kappa B expression and serum IL-6 level following myocardial Ischemia-reperfusion in rats
- VernacularTitle:氯胺酮对心肌缺血/再灌注大鼠心肌NF-?B和血清白细胞介素6的影响
- Author:
Jing WU
;
Yanlin WANG
;
Chengyao WANG
- Publication Type:Journal Article
- Keywords:
Ketamine;
Myocardial reperfusion injury;
NF- kappa B;
Interleukin-6
- From:
Chinese Journal of Anesthesiology
1996;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective Ketamine has been shown to suppress pro-inflammatory cytokines like TNF-? and IL-6. The purpose of this study was to investigate the effects of ketamine on myocardial NF-?B expression and serum IL-6 level in a rat model of myocardial ischemia-reperfusion (I/R) .Methods Twenty-four healthy male SD rats aged 4-8 months weighing 200-250 g were randomly divided into four groups with 6 animals in each group : (A)control group; (B) I/R group; (C) ketamine 5 mg? kg-1 +I/R(I/R+K1) and (D) ketamine 10mg?kg-1 + I/R (I/R + K2) . The animals were anesthetized with intraperitoneal 10% chloral hydrate 40 mg?kg-1. Chest was opened and heart exposed. Left coronary artery was temporarily occluded at 1 mm inferior to left auricle for 30 min followed by 120 min reperfusion. Myocardial ischemia was confirmed by decoloration of apex and elevation of S-T segment. In control group (A) heart was exposed but left coronary artery was not occluded. In group C and D ketamine 5 mg?kg-1 or 10mg?kg-1 was given i.v. during ischemia. The animals were sacrificed at the end of 120 min reperfusion. A piece of myocardium (0.1 g) was obtained from apex for determination of myocardial NF-?B expression (Western-blot) . Blood samples were taken at 30 min and 120 min of reperfusion for determination of serum IL-6 level (ELISA) .Results The serum IL-6 level at 30 min and 120 min of reperfusion and myocardial NF-?B expression were significantly increased in I/R group (B) compared with those in control group ( P
