The Effects of Wortmannin on Leukemia Cells Proliferation Cycle and BCL-2 Expression
- VernacularTitle:Wortmannin抑制PI3K途径对白血病细胞周期特异性及BCL-2蛋白表达影响的研究
- Author:
Zhengfa LI
;
Xiaomei SHEN
;
Renbin ZHAO
- Publication Type:Journal Article
- Keywords:
Cellcycle;
BCL - 2protein;
PI- 3kinase;
Wortmannin;
Apoptosis
- From:
Journal of Chinese Physician
2001;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of wortmannin (WT) on leukemia cells cycle and BCL-2 protein expression. Methods 0 25?mol/L PI-3 kinase inhibitor wortmanin was used to treat K562 cells for 24 hours, and Arac was simultaneously used to induce the cell apoptosis. The specific fluoresent staining and FCM analysis were adopted to measure the cell cycle distribution and BCL-2 expression. Results Compared with the control cells, the proliferation index(PI) of the K562 cells treated by wortmannin was significantly lower, the cell number of G1 phase and the percentage of cell apoptosis increased, and the BCL-2 protein expression significantly decreased. Conclusion Wortmannin could inhibitedtheproliferationofK5 6 2cells ,andwascellcyclespecificagent (CCSA) .
