Construction and Identification of Recombinant Human Bcl-2 Adenoviral Vector
- VernacularTitle:重组人Bcl-2腺病毒载体的构建与鉴定
- Author:
Haibing JU
;
Zaiquan CHENG
;
Zizheng SHU
- Publication Type:Journal Article
- Keywords:
Bcl-2;
Recombinant adenovirus vector;
Plasmid
- From:
Journal of Chinese Physician
2001;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To construct recombinant human Bcl-2 adenoviral vector. Methods Sense human Bcl-2 cDNA fragment from plasimid pcDNA3 1/bcl-2 was cloned into adenovirus shuttle vector pAdCMV. pAdCMV/bcl-2 and plasmid pJM17 were cotransfected into 293 cells with lipofectamine 2000. Replication defective adenovirus was reproduced and purified. Adenoviral virus DNA was detected with polymerase chain reaction(PCR), and plaque assay was performed to determine the titer of virus. Results Recombinant adenovirus containing bcl-2 target gene was obtained. The titer of virus was 2 0?10 10 pfu/ml. Conclusion High titer of adenovirus containing target gene was successfully obtained by recombinant adenovirus vector.
