Influence of simulated microgravity on rat islets morphology in culture
- VernacularTitle:模拟微重力培养大鼠胰岛的形态学观察
- Author:
Chun SONG
;
Xiuqing DUAN
;
Yeqing SUN
;
Xi LI
;
Liou HAN
;
Ping XU
;
Chunfang SONG
;
Lianhong JIN
- Publication Type:Journal Article
- Keywords:
Islets;
Cell culture;
Microscopy,electron
- From:
Chinese Journal of General Surgery
2001;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the influence of simulated microgravity on rat islet. Methods Isolated islet were assigned to flask-culture or bioreactor-culture. Gross structure and ultrastructure of islet were observed by transmission electron microscopy (TEM) and scanning electron microscopy (SEM). Results Islets ultrastructure on 7th day in bioreactor closely resembled fresh islets,with well-formed secretory granules and abundant mitochondria. SEM showed under microgravity islets communicating each other with cavity-like areas. Conclusions The ultrastructure of islets cultured under microgravity closely resembled fresh islets.
