Effects of D-limonene on the adhesive and invasive capability of gastric cancer cell line SGC-7901
- VernacularTitle:右旋柠烯对胃癌细胞粘附和侵袭能力影响的研究
- Author:
Guangming XING
;
Mingyang QU
;
Guiping ZHANG
;
Bingan FENG
- Publication Type:Journal Article
- Keywords:
Stomach neoplasms;
Metalloproteinases;
Tissue inhibitor of metalloproteinases
- From:
Chinese Journal of General Surgery
2001;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effects of D-limonene on the adhesiveness and invasion of gastric cancer cell line-SGC-7901. Methods MTT chromatometry was used to detect the effects of D-limonene on cancer cell′s survival rate. Fibronectin and laminin were employed to detect the adhesive inhibition of SGC-7901 by D-limonene. Using artificial basal membrane,invasion inhibition of SGC-7901 was studied in Transwell cabin. Matrix metalloproteinase 2 (MMP-2) and metalloproteinase 2 (TIMP-2) protein of SGC-7901 was detected by Western-blot. Results The growth of cancer cells was significantly inhibited in a dose dependent manner. The adhesiveness and invasion were prohibited. At the dosage of 0.25 mmol/L,the expression of MMP-2 was down regulated (2.9?0.3),and TIMP-2 up regulated (29.5?0.6) significantly different from control groups (28.6?0.5 and 18.6?0.3,all P
