A study on collagen Ⅳ and MMP-2 expression during the period of venous intima hyperplasia (IH) in rat vein grafts
- VernacularTitle:Ⅳ型胶原蛋白和基质金属蛋白酶2在移植静脉中表达的研究
- Author:
Yang SUN
;
Yanpeng DIAO
;
Tao LUO
;
Qiang ZHANG
- Publication Type:Journal Article
- Keywords:
Proteins;
Metalloproteinases;
Veins;
Transplantation
- From:
Chinese Journal of General Surgery
2001;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate collagen Ⅳ and matrix metalloproteinase-2 (MMP-2)expression in intimal hyperplasia(IH) in rat vein grafts. Methods Vein graft model was constructed in rats. At day 3,week 1,2,4,8 and 12 after surgery,vein grafts were obtained and examined by HE and Verhoeff stains. The thickness and area ratio of intima/media were measured. The positive cells of 5′-Brdu were detected to indicate SMC proliferation. The positive ratio of collagen Ⅳ and MMP-2′s protein and mRNA were calculated. Results The neointima was found at 1 week post-operation. The maximal IH occurred at 2 to 4 week. The similar tendency was observed in the positive cells of 5′-Brdu. Collagen Ⅳ was on the decrease at 1 week and negative from 2 to 8 week ( P
