Relationship Between Expression of MDR_1 Gene in Cochlea Lateral Wall and Accumulation of Adriamycin in Endolymph of Guinea Pig
- VernacularTitle:豚鼠耳蜗外侧壁中MDR_1基因表达与内淋巴液中ADM积聚的关系
- Author:
Yaguang ZHOU
;
Xueyuan ZHANG
- Publication Type:Journal Article
- Keywords:
Lateral wall,cochlea;
MDR 1;
Adriamycin
- From:
Journal of Audiology and Speech Pathology
1998;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the relationship between the expression of MDR 1 gene in cochlea lateral wall and the accumulation of adriamycin in endolymph of guinea pig.Methods Twenty four white guinea pigs with red eyes were randomly chosen,and administered an intravenous injection of 30 mg/kg adriamycin or N.S.The animals were sacrificed 24 hours later,the cochlea lateral wall was then immediately removed after the endolymph was drawn by the siphon from the basal turn of cochlea. The expression of the MDR 1 mRNA in cochlea lateral wall of guinea pigs were observed by RT-PCR.The ADM concentration in endolymph was measured by high performance liquid chromatography.Results The accumulation of adriamycin in endolymph negatively correlated with the expression of MDR 1 gene in cochlea lateral wall(r s=-0.901, P
