Experimental Study on the Apoptosis of HepG_2 Cells Induced by Acivicin or/and Cisplatin
- VernacularTitle:单用Acivicin和联用顺铂诱导HepG_2细胞凋亡的实验研究
- Author:
Weiqing CHEN
;
Dingming SHEN
- Publication Type:Journal Article
- Keywords:
cisplatin;
Acivicin;
apoptosis;
HepG2
- From:
China Pharmacy
1991;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To study the effects of Acivicin or/and cisplatin on the apoptpsis of HepG2 and expression of Bcl-2,c-myc and p53.METHODS:Acivicin and cisplatin were used to treat cultured HepG2 cell line separately or in combination.The cytotoxicity was measured by MTT assay,the apoptosis by TUNEL assay and relevant regulator genes by immunohistochemical SP method.RESULTS:Acivicin in concentrations of 0.28,0.56,0.84,1.12 and 1.40mmol/L,the apoptosis rates of HepG2 were (3?1.25)%,(3.7?2.0)%,(10?1.25)%,(17.4?4.5)%and (16.9?3.5)%respectively.Acivicin in concentration of 1.4mmol/L,the apoptosis rates at 24,48 and 72h were(16.9?3.5)%,(27.9?4.3)%and (47.2?3.0)%respectively,which was significantly different from control group.The apoptosis rate of HepG2 induced by cisplatin(67?mol/L)was (73.4?1.5)%at 24h.The apoptosis rate of HepG2 induced by Acivicin(1.4mmol/L)plus cisplatin(67?mol/L)was(94.7?0.5)%at 24h,which was markedly higher than those induced by Acivicin or cisplatin alone.The expressions of Bcl-2,c-myc and p53 in all groups were increased comparing with those in control group.CONCLUSION:This study indicates that the apoptosis of HepG2 induced by Acivicin was in a dose-dependent and time-dependent manner.When Acivicin and CDDP used simultaneously,the effect of CDDP on HepG2 apoptosis was enhanced greatly.
