Determination of Chlorogenic Acid in Qingyan Granules by RP-HPLC
- VernacularTitle:反相高效液相色谱法测定清炎颗粒中绿原酸的含量
- Author:
Lan CUI
;
Deqiu ZHU
;
Xiaomin WANG
;
Qijian LUO
;
Huiming CAO
- Publication Type:Journal Article
- Keywords:
Qingyan granule;
chlorogenic acid;
RP-HPLC;
content determination
- From:
China Pharmacy
1991;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish a RP-HPLC method for the determination of chlorogenic acid in Qingyan granules.METHODS:Waters Breeze System was used with a Hypersil BDS C18 column(5?m,4.6mm?150mm).The mobile phase was composed of methanol and 0.4%(V/V)H3PO4 water solution(20∶80,V/V),flow rate:1.0ml/min;wavelength of UV detector:327nm,column temperature 20℃.RESULTS:The calibration curve of chlorogenic acid was linear within the range of (174～1 392)ng(r=0.9 999).The average recovery was 100.10%,RSD=1.60%(n=5).CONCLUSION:This method is simple,sensitive,as well as reliable,and is available to quality control.
