Effects of fructose-1,6-diphosphate on adriamycin-induced calcium and sarcoplosnic reticulum Ca ~(2+)-ATPase activity in cardiomyocytes of rats
- VernacularTitle:1,6-二磷酸果糖对阿霉素引起大鼠心肌细胞内游离钙和肌浆网Ca~(2+)-ATP酶活性改变的影响
- Author:
Wei CAI
;
Shan CHEN
- Publication Type:Journal Article
- Keywords:
Fructose-1,6-diphosphate;
Doxorubicin;
Myocardium;
Calcium;
Sarcoplasmic reticulum Ca 2+-ATPase
- From:
Chinese Journal of Pathophysiology
1989;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To study the effects of fructose-1,6-diphosphate (FDP) on adriamycin (ADR)-induced calcium and sarcoplasmic reticulum Ca 2+-ATPase activity in cardiomyocytes of rats. METHODS: Rats were treated with ADR by intraperitoneal injection (2 5 mg?kg -1 body weight) once every two days for 11 days, and then ADR-treated rats were intervened by FDP at different dosages (ip) once every other day for 41 days. Enzyme linked immune absorption assay (ELISA) was employed to detect froponin I (CTnI). CK-MB was examined by monoclonal antibody. Intracellular free calcium concentration was measured on fluorescent spectrophotometry and SRCa 2+-ATPase activity was examined by inorganic phosphate. RESULTS: FDP (300, 600, 1 200 mg?kg -1) significantly reduced the levels of CTnI and CK-MB in serum. Decreased calcium and increased SRCa 2+-ATPase activity in cardiomyocytes were also observed when ADR-treated rats were intervened by FDP (P
