Inhibitory effect of polypeptide from Buthus martensii Karsch on neoplasm cells
- VernacularTitle:蝎毒多肽对肿瘤细胞的抑制作用研究
- Author:
Tianhan KONG
;
Shan LIN
;
Xuefei HAN
;
Jihang JU
;
Weihua DONG
- Publication Type:Journal Article
- Keywords:
Scorpion venoms;
Eca109 cell;
Cell cycle;
Protein P53;
Membrane potentials
- From:
Chinese Journal of Pathophysiology
1989;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To observe the inhibitory effects of polypeptide from Buthus martensii venom (PBMV) on human tumor cell lines and the influence of PBMV on cell cycle migration, expression of tumor-suppressor gene p53 and the membrane potential of CNE-2Z cells. METHODS: MTT colorimetric method, the colony formation and mitosis index, flow cytometry assay and intracellular recording with glass microelectrodes were used. RESULTS: PBMV had obvious cytotoxicity on several tumor cell lines. The cells grow was inhibited by PBMV, colony formation rate and mitotic index of tumor cells were reduced and the number of polykaryocyte was decreased. CNE-2Z cells in S phase were reduced evidently after they were treated with PBMV (P
