Inhibition of proliferation and influence of proto-oncogenes expression by TanshinoneⅡA in U251 cells
- VernacularTitle:丹参酮ⅡA对U251胶质瘤细胞的增殖抑制和原癌基因表达的影响
- Author:
Hui DENG
;
Huanmin LUO
;
Qin GAO
;
Xiaoguang LI
;
Fei XIAO
;
Peifen ZHANG
;
Wen WENG
- Publication Type:Journal Article
- Keywords:
Danshen;
Glioma;
Cell cycle;
Proto-oncogenes
- From:
Chinese Journal of Pathophysiology
1989;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the inhibitory effect of TanshinoneⅡA on U251 glioma cell line and its mechanism. METHODS: MTT was used to measure the levels of the proliferation in U251 cultured with TanshinoneⅡA at different concentrations. The effects of TanshinoneⅡA on cell cycle of U251 were observed by FCM. The expression of proto-oncogene c-myc was measured by RT-PCR. RESULTS: The proliferation of U251 was obviously inhibited by TanshinoneⅡA in a dose dependent manner. The inhibitory rate came to the peak at (54 2?0 9)%, when cultured with TanshinoneⅡA at 0 10 g/L. The outcome of FCM showed that the proportion of G 0/G 1 phase cells was increased and the proportion of S phase cells was reduced obviously, when cultured with TanshinoneⅡA at 0 10 g/L for 3 days. The RT-PCR experiment showed that the expression of proto-oncogene c-myc was notably decreased, when the dose of TanshinoneⅡA was increased. CONCLUSION: TanshinoneⅡA inhibited the proliferation of U251 and the expression of proto-oncogene c-myc.
