Therapeutic action of transplantation of marrow mesenchymal stem cells into infarction area after cerebral middle artery occlusion in rats
- VernacularTitle:间质干细胞移植在大鼠缺血性脑卒中的实验研究
- Author:
Gongxiong WU
;
Zhihua YANG
;
Haiwei ZHANG
;
Yongping LIN
;
Jie XU
;
Chunxiang ZHANG
- Publication Type:Journal Article
- Keywords:
Brain ischemia;
Stem cells;
Cell transplantation
- From:
Chinese Journal of Pathophysiology
1989;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To explore the differentiation and the functional behavior of marrow mesenchymal stem cells (MSC) transplanted into the cerebral infarction area after cerebral middle artery ischemia in rats. METHODS: MSC were isolated from human rib marrow and cultured in L DMEM medium in vitro. The model of rat cerebral infarction by cerebra middle artery occlusion was established, and the identified MSC were transplanted intracerebrally 10 days later. Immunohistochemistry technique was used to identify the cell survivor and its differentiation to the neurogenesis in the transplantation site, and at 2 weeks and 6 weeks after transplantation, the functional tests were comparatively studied. RESULTS: The results showed that the survivor of transplanted MSC was differentiated to neural phenotype cells, and the functional behavior of the injury rats was recovered significantly after MSC transplantation (P
