Construction and sequence analysis of eukaryotic expression vector of Chinese prostate-specific membrane antigen
- VernacularTitle:中国人前列腺特异膜抗原基因真核表达载体的构建和序列测定
- Author:
Kaiyuan CAO
;
Shuqin DAI
;
Lin XU
;
Guangqing YUAN
;
Xiaorong HUANG
;
Shaopeng QIU
;
Linjie GUO
- Publication Type:Journal Article
- Keywords:
Prostate-specific antigen;
Reverse transcriptase polymerase chain reaction;
Cloning, molecular;
Sequence analysis
- From:
Chinese Journal of Pathophysiology
1989;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To obtain eukaryotic expression vector of Chinese prostate-specific membrane antigen. METHODS: Chinese prostate-specific membrane antigen (PSMA) cDNA was amplified by RT-PCR from prostate cancer tissues, then cloned into eukaryotic expression vector pcDNA3 0 and sequenced. RESULTS: Seven bases in Chinese PSMA cDNA sequence were found different from those reported by Israeli, which lead to two different amino acids. CONCLUSION: We have obtained the PSMA cDNA, and the recombinant eukaryotic expression vector was successfully constructed. The study lays foundation for DCs vaccine modified by PSMA gene for the treatment of prostate neoplasms.
