Effect of sodium nitroprusside on activation of nuclear factor ?B
- VernacularTitle:硝普钠对核因子?B活性影响的初步研究
- Author:
Ning ZHANG
;
Yongjian XU
;
Zhenxiang ZHANG
- Publication Type:Journal Article
- Keywords:
Nitroprusside;
NF-kappa B;
T-lymphocytes
- From:
Chinese Journal of Pathophysiology
1989;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the effect of sodium nitroprusside (SNP) on activation of nuclear factor ?B. METHODS: The techniques of culture of human T lymphocytes, Western blot and RT-PCR were applied. The effects of NO donor sodium nitroprusside (SNP) at different concentrations on mRNA and protein expression of I?B ? in human T lymphocytes at 30 min or 120 min after stimulating with phytohaemagglutinin (PHA-P) were observed. RESULTS: SNP at middle or high concentrations reduced the degradation of I?B ? protein 30 min after stimulating with PHA-P, and increased the re-expression of I?B ? mRNA 120 min after stimulating with PHA-P significantly. CONCLUSION: The mechanism of inhibitory effect of SNP at middle or high concentrations may be due to the decrease in degradation and the increase in re-synthesis of I?B ?. The regulatory mechanism of SNP at low concentration may not be through I?B ?.
