Reconstructed corneal endothelial tissue on allo-stroma in vitro
- VernacularTitle:脱水猪角膜基质为载体体外构建猫角膜内皮组织
- Author:
Jing WU
;
Shuyan LI
;
Nian HAO
;
Jintang XU
;
Songbin ZHAO
- Publication Type:Journal Article
- Keywords:
Endothelium, corneal;
Corneal stroma;
Swine;
Cats
- From:
Chinese Journal of Pathophysiology
1989;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: In order to search carrier material and better tissue culture method, the morphology and structure of the cultured cat corneal endothelium was observed. METHODS: The cat corneal endothelial cells were seeded on the Descemet's membrane of the dehydrated swine corneal stroma, and then cultured in the medium with epidermal growth factor and laminin for 7 days. The morphology and structure of reconstructed tissue was tested by the inverted microscope and the scanning electron microscope. RESULTS: As shown by the morphological observations, the cultured cat corneal endothelial cells formed an integrated membrane, and attached to the Descemet's membrane, which was similar to the nature tissue. The cells connected tightly to each other, and some of them arranged in hexagon approximately. CONCLUSION: The cat corneal endothelial cells could be rebuilt on the carrier of the dehydrated swine corneal stroma successfully, which is similar to the nature tissue.
