IgA_1 from patients with IgA nephropathy induced release of calcium, up-regulation of TGF-? mRNA expression and secretion of fibronectin in human glomerular mesangial cells
- VernacularTitle:IgA肾病患者血清IgA_1诱导正常人肾小球系膜细胞钙离子释放、转化生长因子?表达上调和纤连蛋白分泌
- Author:
Yue WANG
;
Minghui ZHAO
;
Youkang ZHANG
;
Xiaomei LI
;
Haiyan WANG
- Publication Type:Journal Article
- Keywords:
Glomerulonephritis, IGA;
Immunoglobulin A;
Calcium;
Transforming growth factor beta;
Glomerular mesangial cells
- From:
Chinese Journal of Pathophysiology
1989;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To study and compare the pathophysiological effects of serum IgA 1 from both the patients with IgA nephropathy (IgAN) and healthy controls on human mesangial cells (HMC). METHODS: Serum IgA 1 was isolated with Jacalin affinity chromatography and heated to form aggregates (aIgA 1). Primary HMC were cultured and passage 3 and passage 4 of the cells were used. Intracellular calcium release was assayed with confocal analysis. Expression of TGF-? mRNA and the content of supernatant fibronectin were tested by RT-PCR and indirect competitive ELISA, respectively. RESULTS: aIgA 1 from patients with IgAN was shown to induce release of intracellular calcium, up-regulation of expression of TGF-? mRNA and secretion of fibronectin in HMC in a similar time-dependent manner as aIgA 1 from healthy controls, but the effects of the former were much stronger and the durations was much longer (P
