Effect of ginsenoside Rg1 on the amyloid protein precursor and neprilysin expression induced by lipopolysaccharide in C6 cell line
- VernacularTitle:人参皂苷Rg1对脂多糖诱导的C6细胞株淀粉样前体蛋白和脑啡肽酶表达的影响
- Author:
Huanmin LUO
;
Hui DENG
;
Feng HUANG
;
Fei XIAO
;
Qin GAO
;
Xiaoguang LI
- Publication Type:Journal Article
- Keywords:
Ginseng;
Lipopolysaccharides;
Glioma;
Amyloid beta-protein precursor;
Neprilysin
- From:
Chinese Journal of Pathophysiology
1989;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: This study was designed to investigate the effect of ginsenoside Rg1 on the amyloid ?-protein precursor (APP) and neprilysin (NEP)expression induced by lipopolysaccharide (LPS) in C6 cell line in order to discover effectual Alzheimer's disease (AD)-treated drugs. METHODS: MTT colorimetric analysis was used to measure the survival rate of C6 cultured with ginsenoside Rg1 at different concentrations (2 5, 5, 10 and 20 ?mol?L -1) and LPS (100 mg?L -1). The expression of APP and NEP mRNA was measured by RT-PCR. RESULTS: LPS decreased the survival rate of C6, furthermore, the increase in APP expression and the decrease in NEP expression were observed. On the other hand, the above alteration induced by LPS was reversed by ginsenoside Rg1. CONCLUSION: This study demonstrates that LPS can cause cell damage, the increase in APP expression and the decrease in NEP expression. Ginsenoside Rg1 can exert a neuroprotective action, protect C6 cells against LPS-induced injury via inhibiting APP expression and increasing NEP expression.
