Effects of Keningfang decoction on heat shock protein 70 expression in lung of mice with influenza virus pneumonia
- VernacularTitle:咳宁方对流感病毒性肺炎小鼠肺组织HSP70表达的影响
- Author:
Qinhe YANG
;
Xuechun TANG
;
Xiaoyin CHEN
;
Shengquan PENG
;
Xuemei CHEN
;
Bingde LUO
;
Jingbo SUN
;
Yunsheng XU
;
Min MA
- Publication Type:Journal Article
- Keywords:
Keningfangs decoction;
Pneumonia, viral;
Heat shock-proteins 70
- From:
Chinese Journal of Pathophysiology
1989;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To study the preventive effects of Keningfang decoction on the experimental influenza virus pneumonia in mice and its mechanism. METHODS: Fifty NIH mice were divided into five groups randomly (ten mice in each group), normal control group, model group, virazole treatment group, Keningfang I treatment group, Keningfang II treatment group. The FM 1 virus strain that kept in frozen condition were revived and cultured in chick embryo. The mice in every group that were lightly anesthetized by aether, and infected by dropping FM 1 15 LD 50 into the nose, except for the normal control group, by equal volume distilled water. Mice were treated with drugs or distilled water two days before the model was made (0 3 mL, 2 times a day). The mice were treated with drug for six days, then was killed, the lungs were collected, and kept in -70 ℃. HSP70 was measured in the lung tissue by Western blot. Pathologic changes of the mice lungs were observed under microscope. RESULTS: Compared with the normal control group, HSP70 in the other groups were increased significantly (P
