High glucose induces lectin-like oxidized LDL receptor-1 expression in endothelial cells
- VernacularTitle:高糖诱导内皮细胞氧化型低密度脂蛋白受体表达
- Author:
Li XUE
;
Jiang SHAN
;
Geng XU
;
Xuehua ZHANG
- Publication Type:Journal Article
- Keywords:
Glucose;
Endothelial cells;
Receptors, LDL
- From:
Chinese Journal of Pathophysiology
1989;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To explore the effects of high D-glucose on the mRNA and protein expression of lectin-like oxidized LDL receptor-1 (LOX-1) in endothelial cells and to study the possible mechanism by which diabetes is easily accompanied by vascular diseases. METHODS: Cell morphology was observed with inverted phasecontrast microscope. LOX-1 mRNA was detected by quantitative reverse transcription polymerase chain reaction in vascular epithelial cells cultured with different concentration D-glucose (7 5, 15 or 30 mmol/L) or with the same concentration D-glucose (15 mmol/L) in different time (0, 12, 24, 48, 72 or 96 h), LOX-1 protein expression was also examined by immunohistochemisty. RESULTS: Cells, exposed to high concentration D-glucose, were crude with clear profiles and bright granules in the plasma. Different high concentration D-glucose could induce LOX-1 mRNA expression and the effect of 30 mmol/L glucose on LOX-1 mRNA was the most significant (P
