DNA Microarray Probe Preparation Using cDNA Fragments Library from HCV Genotype 1
- VernacularTitle:应用cDNA文库法制备HCV-1诊断芯片探针
- Author:
Zhaohui SUN
;
Wenling ZHENG
;
Bao ZHANG
- Publication Type:Journal Article
- Keywords:
HCV;
Clone;
Microarray;
Gene probes
- From:
Journal of Chinese Physician
2001;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the feasibility of preparing HCV-1 diagnostic microarray probes using the technique of cDNA fragments library construction. Methods The full-length cDNAs of HCV of subtypes 1a and 1b were digested with restriction endonuclease Sau3A I, and the resulted fragments were cloned into the pMD18-T vector. Positive clones were isolated and identified by sequencing. Results A total of 57 different fragments were obtained, and sequence analysis showed that all the fragments ranging from 200 to 1000bp were specific gene fragments of HCV genotype 1, which can be efficiently used as probes in microarray prepapration. Conclusion The method of preparing microarray probes by construction of cDNA fragments library was effective, quick and simple.
