Real-time Quantitative Assay of Insertion Sequence 6110 DNA of Mycobacterium Tuberculosis and Its Value for Diagnosis of Tuberculosis
- VernacularTitle:结核分枝杆菌插入序列6110DNA实时定量检测及其对结核病诊断价值的探讨
- Author:
Xiang HUANG
;
Yirong LI
;
Zhengxue WU
- Publication Type:Journal Article
- Keywords:
Mycobacterium tuberculosis;
Insertion sequence;
Polymerase chain reaction
- From:
Journal of Chinese Physician
2001;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To set up a real-time quantitative assay method of insertion sequence 6110 DNA of mycobacterium tuberculosis, and explore its value for diagnosing tuberculosis. Methods Real-time quantitative assay of insertion sequence 6110 DNA of mycobacterium tuberculosis was performed with Taqman technique and Lightcycler quantitative PCR system. Results 213 clinical samples of different types were detected, 32 cases were positive, and the positive rate was 15 02%. The scope of quantitative results of positive samples was 3 1～7 2?10 6 copies/ml, and the sensitivity and specificity for diagnosis of tuberculosis were 82 76% and 95 65% respectively Conclusion Real-time quantitative assay of insertion sequence 6110 DNA of mycobacterium tuberculosis is a rapid and effective method for diagnosis of tuberculosis.
