The Mutation of embB and pncA Genes in Drug-resistanct Mycobacterium Tuberculosis and Its Clinical Value
- VernacularTitle:结核菌耐药pncA和embB基因突变与临床治疗的研究
- Author:
Hongmin LI
;
Xueqiong WU
;
Wei WANG
- Publication Type:Journal Article
- Keywords:
Mycobacterium tuberculosis;
Drug resistance;
PCR-SSCP
- From:
Journal of Chinese Physician
2001;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the mutation of embB and pncA genes in mycobacterium tuberculosis isolates, and assess its clinical value. Methods The drug-sensitivity of 106 clinical isolates of mycobacterial species was identified by the tranditional method, and then analyzed the mutation of their embB and pncA genes with PCR-SSCP. Mycobacterium tuberculosis strain H 37 RV was used as a control. Results The mutational frequency of pncA gene in 94 PZA-resistant mycobacterium tuberculosis was 44 6%(42/94). The mutational frequency of embB gene in 83 EMB-resistant isolates was 54 2%. The frequency of both genes mutation was 11 8%(11/94). Conclusion EMB and PZA resistances in some mycobacterium tuberculosis isolates were due to mutations of embB and pncA genes. PCR- SSCP method might become a simple and rapid diagnostic test for genotypes of EMB and PZA resistant mycobacterium tuberculosis.
