Atorvastatin Reduces the LPS-induced COX-2 Expression in Cultured Human Pulmonary Epithelial Cells
- VernacularTitle:阿托伐他汀降低脂多糖诱导下人肺上皮细胞COX-2的表达
- Author:
Shangjie WU
;
Ying CAI
;
Shuiping ZHAO
- Publication Type:Journal Article
- Keywords:
Atorvastatin;
Human pulmonary epithelial cells lines (A549);
Cyclooxygenase-2 (COX-2)
- From:
Journal of Chinese Physician
2001;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To assess the effect of atorvastatin on the Lipopolysaccharide (LPS)-induced cyclooxygenase-2 (COX-2) expression in cultured human pulmonary epithelial cells (A549). Methods A549 cells were incubated in the medium containing LPS and different concentrations of atorvastatin(0,10,15,20?M/ml, respectively) for 12h. Then the total cellular RNA and proteins from the cells treated with different experimental conditions were extracted for RT-PCR and western blot analysis,respectively. Results In cultured human pulmonary epithelial cells, atorvastatin reduced the expression of COX-2 mRNA and protein induced by LPS in a dose-dependent manner. Conclusion Atorvastatin may down-regulate LPS-induced COX-2 expression in cultured human pulmonary epithelial cells.
