The Protective Effect of Naoyian on Rat Cerebral Microvascular Endothelial Cells Lmpairment Induced by Hydrogen Peroxide
- VernacularTitle:脑溢安对过氧化氢损伤大鼠脑微血管内皮细胞的保护作用
- Author:
Yihui ZHI
;
Xingqun LI
;
Yun LUO
- Publication Type:Journal Article
- Keywords:
Microvascular endothelial cells;
Hydrogen peroxide;
Rat;
Naoyian;
PDTC
- From:
Journal of Chinese Physician
2001;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a hydrogen peroxide-impaired model of rat cerebral microvascular endothelial cells in vitro, and observe the protective effect of serum containing Naoyian. Method Rat cerebral microvascular endothelial cells were treated with six concentrations of hydrogen peroxide at four time points. The optimum injury condition of hydrogen peroxide was determined by MTT chromatometry. Then the cultured cells pretreated with serum containing Naoyian and pyrrolidine dithiocarbamate(PDTC) were interfered with hydrogen peroxide in suitable concentration and time, and OD of the treated cells was measured by MTT chromatometry. Results The cultured cells were injured obviously by hydrogen peroxide in 0 125mmol/L for 30min. The OD of the cells pretreated with 5% serum containing Naoyian and 50?mol/L PDTC were significantly higher than that of the cells without pretreatment (P
