Effects of Clonidine-Displacing Substance on the Function of Isolated Islets from Normal Rats
- VernacularTitle:可乐定置换物对正常大鼠离体胰岛功能的影响
- Author:
Xiaogang WENG
;
Jingfang DOU
;
Xiaohui GONG
- Publication Type:Journal Article
- Keywords:
Clonidine-Displacing Substance(CDS);
Rats;
Isolated islets;
Insulin;
Glucagon
- From:
Journal of Chinese Physician
2001;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effects of the clonidine-displacing substance(CDS)on the function of isolated islets from normal rats. Methods The SD rat islets were isolated by digestive method with collagenase and DNase, and incubated in RPMI 1640 culture medium overnight. Multiscreen Assay System was used to observe the effects of CDS on the function of the isolated islets. Insulin and glucagon were measured by RIA. Results Glucagon secretion of the isolated islets in incubation medium was inhibited by glucose. CDS could significantly inhibit glucagon secretion in concentration-dependent manner. Insulin secretion of the isolated islets was dependent on the glucose concentration of incubation medium. CDS could stimulate insulin secretion significantly. Nifedipine (Ca 2+ channel blocker) and diazoxide (K + channel opener) could inhibit the insulin release of the isolated islets, the effect of which could be abolished by CDS. Conclusion As an endogenous ligand of imidazoline receptors, CDS could stimulate insulin secretion, and inhibit glucagon release in the isolated islets.
