All-Trans-Retinoid Effecting Endothelial Cell Migration and Gelatinase Expression
- VernacularTitle:全反式维A酸对内皮细胞迁移能力及明胶酶表达的影响
- Author:
Hui DENG
;
Chunlin YAN
;
Shuxian YAN
;
Yue HU
;
Yu XU
;
Kanghuang LIAO
- Publication Type:Journal Article
- Keywords:
Tretinoin;
Endothelium;
Gelatinase A;
Gelatinase B;
Cell, cultured
- From:
Chinese Journal of Dermatology
1994;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of all-trans-retinoid(ATRA) on the migration of endothelial cells and the expression of gelatinases. Methods In vitro migration assay was used to determine the effect of ATRA on the endothelial cell migration induced by phorbol 12-myristate-13-acetate (PMA), semi-quantitative RT-PCR and Western blot were used to observe the effect of ATRA on the gelatinase expression at mRNA and protein levels respectively, while the proteolytic activities of gelatinases were assessed by zymography. Results The endothelial cell migration elicited by PMA was significantly inhibited when incubated with 0.1 ?mol/L, 1.0 ?mol/L and 10.0 ?mol/L ATRA. Compared with the control, the inhibition rates were (44.68 ? 7.79)%, (65.20 ? 4.59)% and (78.37 ? 2.58)%, respectively. ATRA also reduced the expression and activities of gelationases in a dose dependant manner. At 10 ?mol/L concentration, the inhibition rate of mRNA expression, protein expression and protein activities for gelatinase A was (59.39 ? 7.98)%, (78.40 ? 3.23)% and (53.02 ? 7.23)%, respectively. For gelatinase B it was (65.23 ? 3.62)%, (82.49 ? 2.88)% and (47.32 ? 7.72)%, respectively. Conclusions The expression and activities of gelatinases are downregulated in the endothelial cells when incubated with ATRA, which may be the possible mechanism of ATRA inhibiting the endothelial cell migration elicited by PMA in vitro.
