Effects of ketamine on lipopolysaccharide-induced activation of human umbilical vein endothelial cells
- VernacularTitle:氯胺酮对脂多糖诱导下人脐静脉内皮细胞活化的影响
- Author:
Haiyun WANG
;
Guolin WANG
- Publication Type:Journal Article
- Keywords:
Ketamine;
Lipopolysaccharides;
Intercellular adhesion molecule-1;
NF-kappa B;
Endothelium, vascular;
Umbilical veins
- From:
Chinese Journal of Anesthesiology
1994;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objectivc To study the effects of ketamine and MK-801 on lipopolysaccharide (LPS)-inducedexpression of intercellular adhesion molecule-1 (ICAM-1 ) and the translocation of nuclear factor-kappa B (NF-?B)into nuclei of human umbilical vein endothelial cells (HUVECs). Methods The endothelial cells obtained fromhuman umbilical vein by digestion with collagenase 1 were cultured at 37℃ in a 5 % CO_2-95% room airatmosphere for 7 days. The cultured human umbilical vein endothelial cells (HUVECs) wer randomly divided into(1) control group in which nothing was added to the RPMI-1640 culture medium; (2) LPS group in which us 1?g?ml~(-1) was added to RPMI-1640; (3) ketamine group was further divided into 4 subgroups according to theconcentration of ketamine added: 12.5 (KⅠ), 25 .0 (KⅡ), 100 (KⅢ) and 300?mol?L~(-1) (KⅣ); (4) MK-801group was further divided into 4 subgroups M Ⅰ-Ⅳ, according to the different concentrations of MK-801 added(1 .25, 2.50, 10, 30?mol?L~(-1)). In one set of experiment in ketamine and MK-801 groups after addition ofketamine and MK-801 to the RPMI-1640 culture medium, the HUVECs were incubated for 30 min at 37℃, thenLPS1?g?ml~(-1) was added and incubated for 18 h in a 5 % CO_2 atmosphere at 37℃. The HUVECs were thenharvested for determination of ICAM-1 expression by flow cytometry. In another set of experiment the culturedHUVECs were stimulated with LPS 1?g?ml~(-1) for 2 h in the absence or presence of ketamine (12.5, 25 .0, 100and 300?mol?L~(-1) ) or MK-801 (1.25, 2.50, 10 and 30?mol?L~(-1)). The translocation of NF-?B p65 into nucleiwas detected by immunohistochemistry technique. Results In group K Ⅱ, KⅢ and KⅣ the upregulation ofICAM-1 and translocation of NF-?B into nuclei of HUVECs induced by LPS were significandy reduced (P0.05). Conclusion Ketamine can suppress LPS- induced HUVEC activation at the higher concentrations (≥25?mol?L~(-1)). The inhibitory effects of ketamine ismost likely not mediated through NMDA receptor interactions.
