The protective mechanism of insulin-like growth factor-1 from hypoxic-ischemic brain damage in neonatal rats
- VernacularTitle:胰岛素样生长因子1对新生大鼠缺氧缺血性脑损伤保护机制的研究
- Author:
Lian JIANG
;
Jian CHEN
;
Gang KUANG
- Publication Type:Journal Article
- Keywords:
Hypoxia-ischemia,brain;
Insulin-like growth factor 1;
Glutamic acid;
Apoptosis;
Proto-oncogene proteins c-bcl-2
- From:
Chinese Journal of Perinatal Medicine
1998;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the protective effect of insulin-like growth factor-1 (IGF-1) on hypoxic-ischemic brain damage (HIBD) in newborn rats. Methods One hundred and twenty 7-day-old Wistar rats were randomly divided into sham control,HIBD,HIBD treated with 0.2 mg/kg RH-IGF-1 ,HIBD treated with 0.066 mg/kg RH-IGF-1,and normal saline control groups. Those groups were further divided into 24 h、 48 h and 72 h groups respectively after damage with 8 rats in each group. The histological feature,levels of glutamate (Glu) and apoptosis,and expression of Bcl-2 protein in brain were observed in each group. Results (1) Level of Glu in HIBD 48 h group (1162.2?108.1) mg/kg and levels of apoptosis in every HIBD groups[24 h: (7.6?1.9),48 h: (12.6?1.2),72 h: (13.8?0.9) %] are significantly higher than those in sham groups of the same time,[Glu: (750.9? 53.4) mg/kg and apoptosis: 24 h: (2.0?0.2)%,48 h: (2.0?0.3)%,72 h: (2.0? 0.2)%] respectively ( P
