Role of Akt/NF-?B pathway in immune-complexes-induced MCP-1 and CSF-1 expression in murine glomerular mesangial cells
- VernacularTitle:Akt/NF-?B信号途径参与免疫复合物诱导肾小球系膜细胞表达趋化因子
- Author:
Bifei WANG
;
Hanshi XU
;
Youji LI
;
Yujie LI
;
Rengao YE
;
Xueqing YU
- Publication Type:Journal Article
- Keywords:
Akt kinase;
NF-kappa B;
Antigen-antibody complex;
Monocyte chemoattractant protein-1;
Macrophage Colony-stimulating factor
- From:
Chinese Journal of Pathophysiology
1989;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To explore the role of Akt/NF-?B pathway in immune-complexes-induced monocyte chemoattractant protein-1 (MCP-1) and colony stimulating factor-1 (CSF-1) expression in Mesangial Cells. METHODS: Primary murine glomerular mesangial cells were cultured in vitro and divided into control group, stimulation group and antisense, sense and mismatched oligodeoxynucleotide group. In control group, the cells were stimulated with monomeric IgG after treatment with 0.5% lipofectin for 8 h. In stimulation group, the cells, which had been treated with 0.5% lipofectin for 8 h, were stimulated with aggregated IgG. In antisense, sense and mismatched oligodeoxynucleotide group, being transduced antisense, sense and mismatched oligodeoxynucleotide respectively with 0.5% lipofectin 8 h, the cells were stimulated with AIgG. MCP-1 and CSF-1 in supernatant were deteced with ELISA. In addition, RT-PCR was used to determine MCP-1 and CSF-1 mRNA expression, and EMSA to investigated the activation of NF-?B. RESULTS: Mesangial cells cultured in vitro had a low level NF-?B activation and a low level constitutive expression of MCP-1 and CSF-1. Stimulated with AIgG, activation of NF-?B was markedly increased(0.35?0.06 vs 0.75?0.16, P
