Antiproliferative effect of c-myc antisense oligonucleotide in rat thymus lymphocytes
- VernacularTitle:反义c-myc寡核苷酸抑制大鼠胸腺淋巴细胞增殖
- Author:
Yingge LIU
;
Haowen QI
;
Huanzhang LI
;
Mingquan SU
;
Wenbin YU
;
Yueyun MA
- Publication Type:Journal Article
- Keywords:
Genes, myc;
Oligonucleotides, antisense;
Lymphocytes
- From:
Chinese Journal of Pathophysiology
1989;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To observe the antiproliferative effect of c-myc antisense oligonucleotide in rat thymus lymphocytes. METHODS: Rat thymus lymphocytes were separated by Ficoll-Urografin density gradient centrifugation. Lipofectin was used to introduce antisense, sense and mismatched oligonucleotides for c-myc to rat thymus lymphocytes. The antiproliferative effect was assayed by incorporation of -TdR and MTS cell proliferation assay. TR-PCR was used to detect the expression of c-myc mRNA. RESULTS: c-myc antisense oligonucleotide inhibited rat thymus lymphocytes proliferation [(0.14?0.03)A vs (0.32?0.16)A, P
