Effects of hydrogen peroxide on sodium current in acutely isolated rat hippocampal CA1 neurons
- VernacularTitle:过氧化氢对大鼠海马CA1区神经元钠电流的影响
- Author:
Ziqiang MENG
;
Aifang NIE
- Publication Type:Journal Article
- Keywords:
Hippocampus;
Neurons;
Patch-clamp techniques;
Hydrogen peroxide;
Na+ current
- From:
Chinese Journal of Pathophysiology
1989;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM and METHODS: The effects of hydrogen peroxide on Na+ currents were studied in freshly dissociated rat hippocampal CA1 neurons using the whole-cell patch-clamp techinique. RESULTS: 2貶 2O 2 caused a dose-dependent and voltage-dependent increase in the voltage-activated Na+ currents. The amplitudes of Na+ currents were increased (48.0?4.2)% and (88. 2?5. 1)% (n=10) by H 2O 2 at 10 ?mol/L and 100 ?mol/L, respectively. ②H 2O 2 (10 ?mol/L) did not affect the activation process, but changed the inactivation process significantly. Before and after application of 10 ?mol/L of H 2O 2, the half-inactivation voltage was (-64.58?1.22)mV and (-53.55?0.94)mV (n=10, P
