Selection of targeted glioblastoma tumor cell-binding and internalizing peptides through phage display vector
- VernacularTitle:通过噬菌体载体筛选胶质瘤细胞结合的内化短肽
- Author:
Bing WANG
;
Xueyun ZHONG
;
Yanfang QIN
;
Ying ZHONG
;
Lina YU
- Publication Type:Journal Article
- Keywords:
Glioblastoma;
Peptide library
- From:
Chinese Journal of Pathophysiology
1989;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To isolate peptides targeted binding and internalizing into glioblastoma cell line SWO-38. METHODS: Tumor cells were screened five rounds of whole cell screen through the Ph.D.-12 phage display library. The monoclone specific binding efficiency to the tumor cell was analyzed, and the DNA of phages were extracted, sequenced and translated to the sequences of amino acid. RESULTS: In the phage library after five rounds of screen , 10 of 13 monoclones had highly selective binding to SWO-38 cells. We found two repeated peptide sequences. CONCLUSION: Whole cell screening against tumor cells through random phage peptide library can obtain phage peptides with highly specific binding and internalizing ability. The peptides could be used as a therapy vector for tumor targeted delivery.
