Effects of nuclear factor-kappa B decoy oligodeoxynucleotides on generation of nitric oxide and reactive oxygen species in lipopolysaccharide-induced mouse macrophages
- VernacularTitle:NF-?B“decoy”寡核苷酸对LPS诱导巨噬细胞产生NO及活性氧的影响
- Author:
Jian WANG
;
Jinbao LI
;
Xuyi REN
;
Xiaoming DENG
- Publication Type:Journal Article
- Keywords:
Macrophages;
NF-?B;
Oligonucleotides;
Nitric oxide;
Nitric-oxide synthase;
Reactive oxygen species
- From:
Chinese Journal of Pathophysiology
1989;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To study the effects of nuclear factor-kappa B decoy oligodeoxynucleotides on generation of nitric oxide and activated oxygen in lipopolysaccharide-induced mouse macrophages.METHODS: Mouse macrophage cell lines J774.1 cells were cultured with lipopolysaccharide and liposome-mediated oligodeoxynucleotides, and the level of nitric oxide, activated oxygen and inducible nitric oxide synthase were measured. RESULTS: Nuclear factor-kappa B “decoy”oligodeoxynucleotides decreased the activity of inducible nitric oxide synthase in lipopolysaccharide-induced macrophage, inhibited generation of nitric oxide and reactive oxygen species. The level of nitric oxide, activated oxygen and inducible nitric oxide synthase did not change in control group.CONCLUSION: Nuclear factor-kappa B “decoy”oligodeoxynucleotides decrease the generation of nitric oxide and activated oxygen in lipopolysaccharide-induced macrophages, which is probably due to inhibiting the binding sites of activated nuclear factor-kappa B specially.
