Internal standards for mRNA quantitative expression in hypoxic astrocytes
- VernacularTitle:缺氧时星形胶质细胞定量mRNA表达的内部参照标准研究
- Author:
Anding XU
;
Shaohua TAN
;
Yang QU
- Publication Type:Journal Article
- Keywords:
Astrocytes;
Anoxia;
Gene expression;
Glyceraldehyde-3-phosphate dehydrogenase;
?-actin;
Endothelin converting enzyme-2
- From:
Chinese Journal of Pathophysiology
1989;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the effect of hypoxia on glyceraldehyde-3-phosphate dehydrogenase (GAPDH), ?-actin and endothelin-converting enzyme (ECE)-2 mRNA levels in cultured mouse brain astrocytes (AC). METHODS: AC from neonatal mouse brain was incubated for 24 h in serum-free medium under hypoxic or normoxic conditions. The amount of transferred RNA was estimated using ethidium bromide stained 28S rRNA and 18S rRNA. The levels of tested mRNA were evaluated by Northern blot RNA hybridization. RESULTS: The GAPDH mRNA was up-regulated to 503.0% of the normoxic controls in hypoxic AC (n=10. P
