rhG-CSF increases the expression of fibronectin in the marginal zone of the cerebral infarction in rats
- VernacularTitle:重组人粒细胞集落刺激因子增加脑缺血周边区纤维结合蛋白的表达
- Author:
Yanxin ZHAO
;
Chuanzhen LV
;
Yumin LUO
- Publication Type:Journal Article
- Keywords:
Granulocyte colony stimulating factor, recombinant;
Bone marrow stem cells;
Fibronectins;
Brain ischemia
- From:
Chinese Journal of Pathophysiology
1989;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To explore whether the administration of rhG-CSF improve the neurological function after focal cerebral ischemia and observe the expression of fibronectin. METHODS: Injection of 10 ?g?kg -1?d -1 rhG-CSF for 5 days was given subcutaneously to rats undergoing middle cerebral artery occlusion (MCAO). Neurological severity scores (NSS) test was performed. The expression of fibronectin and Brdu were observed by immunohistochemistry. To visualize the cellular colocalization of Brdu and fibronectin, glial fibrillary acidic protein (GFAP) and fibronectin, double fluorescent staining were used. RESULTS: ① NSS at 7th day, 14th day and 21th day of groups undergoing MCAO treated with rhG-CSF were 4.00?0.89, 3.83?1.17, 3.50?1.38, respectively. NSS at 7th day, 14th day and 21th day of the control groups undergoing MCAO were 5.50?1.38, 5.83?1.47,5.66?1.63, respectively. NSS of the treated groups were significantly lower than that of the control groups (P
