Radioligand binding assay of insulin receptor in rabbit kidney during ischemia and reperfusion
- VernacularTitle:家兔缺血再灌注肾组织胰岛素受体的分析
- Author:
Tao HE
;
Huafu XIE
;
Chenggang DUAN
;
Ronghui LI
;
Zhiqiang FENG
- Publication Type:Journal Article
- Keywords:
Receptor, insulin;
Reperfusion;
Rabbits;
Kidney
- From:
Chinese Journal of Pathophysiology
1989;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To observe the change of insulin receptor in rabbit kidney with acute ischemic-reperfusion injury. METHODS: 15 Japanese white rabbits were allocated randomly into control group, ischemic-reperfusion group(IR group). IR group received clamping for 1 h followed by 2 h or 48 h of reperfusion. At 2 h or 48 h after reperfusion, glucose and insulin in serum were determined. Insulin receptor in renal tissue was analyzed by radioligand binging assay(BAD). RESULTS: The level of serum glucose increased after 2 h reperfusion in 2 groups, but in IR group the value increased much more higher than those in control groups(P
