A Study on the Effects of Interferon-alpha on Hepatitis B virus in Vitro
- VernacularTitle:干扰素?抗乙型肝炎病毒作用的体外实验研究
- Author:
Yongfeng YANG
;
Deming TAN
;
Yutao XIE
- Publication Type:Journal Article
- Keywords:
Interferon;
HepG2 2 2 15 cells;
Hepatitis B virus;
Inhibition
- From:
Journal of Chinese Physician
2001;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the in vitro inhibiting activities of interferon-alpha (INF-?) against hepatitis B virus (HBV). Methods HepG2 2 2 15 cells were treated with INF-?. At 3rd, 6th and 9th days after treatment, the supernatant was collected for HBsAg quantitative assay, and total RNA of the cells was isolated for RT-PCR and realtime-PCR assay of HBV mRNA. Results There were no significant differences in HBsAg titer and virus mRNA level at 3rd and 6th days between the experimental and control groups. At 9th day, HBsAg titer and virus mRNA level in INF-? group were significantly lower than those in control group. And 500IU/ml INF-? could get maximal inhibiting effect against HBV. Conclusion INF-? can inhibit transcription and expression of HBV gene in HepG2 2 2 15 cells, which indicated that INF-? can inhibit HBV replication directly.
