Expression of T cell receptor V_?8.3 gene in rats with experimental autoimmune uveoretinitis
- VernacularTitle:实验性自身免疫性葡萄膜视网膜炎大鼠T细胞受体V_?8.3基因的表达
- Author:
Rui ZHANG
;
Peizeng YANG
;
Liqun CHU
- Publication Type:Journal Article
- Keywords:
Uveal Retinitis;
Receptors, antigen, T-cell;
Gene expression;
Polymerase chain reaction;
Disease models, animal
- From:
Chinese Journal of Ocular Fundus Diseases
2001;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression of T cell receptor (TCR) V ?8.3 gene on CD4+ T lymphocytes in the rats with experimental autoimmune uveoretinitis (EAU). Methods Eighteen Lewis rats were divided into EAU, complete Freund′s adjuvant, and the control group. Interphotoreceptor retinoid-binding protein (IRBP) R16 peptide was synthesized using Fmoc procedure for induction of EAU. Magnetic absorption cell sorting (MACS) method was used to isolate the CD4+ T lymphocytes from the spleen of the rats. Flow cytometry was used to monitor the efficiency of isolation. The expression of TCR V ?8.3 gene segment on CD4+T lymphocytes was determined by fluorescent quantitative polymerase chain reaction. Results EAU was successfully induced in the Lewis rats immunized with IRBP R16 peptide. The proportion of CD4+ T lymphocytes isolated by means of MACS was statistically higher than that before isolation (P
