A study on differently expressed genes in primary varicose vein by cDNA microarray
- VernacularTitle:用基因表达谱芯片筛选原发性下肢静脉曲张相关基因的研究
- Author:
Xinhua HU
;
Jun YANG
;
Gefei LIU
;
Qiang ZHANG
- Publication Type:Journal Article
- Keywords:
Varicose veins;
Gene expression;
cDNA microarray
- From:
Chinese Journal of General Surgery
1994;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To screen the differently expressed genes in great saphenous varicose vein using cDNA microarray. Methods Varicose veins from 5 cases and normal veins near the saphenofemoral junction were collected, total RNA was isolated and mRNA was purified by oligotex. mRNA from varicose veins and normal veins were reversely transcribed to cDNA with the incorporation of fluorescent dUTP to prepare the hybridization probes, which were hybridized to cDNA microarray of 4 096 human genes. RT-PCR and Western blot were used to identify differently expressed genes. Results One hundred and sixty-eight genes were shown in differently expressed profile with 96 upregulated and 72 downregulated genes, in all 5 varicose samples there were 28 genes upregulated and 11 downregulated. The differently expressed genes were involved in the functions of apoptosis, signal conduct, protooncogene and anti-oncogene. The expression of caspase 9 and MAP3K were identified by RT-PCR and Western blot. Conclusion Disease-related gene screening by cDNA microarray in great saphenous varicose vein affords an insight into the pathogenesis of varicosis.
