Construction and identification of adenovirus vector containing mouse CD40Ig gene
- VernacularTitle:携带小鼠CD40Ig基因重组腺病毒的构建及鉴定
- Author:
Tian XIA
;
Xinghua GOU
;
Lnan YAN
;
Dehua LI
;
Haiyang HU
;
Juan YAN
;
Bo LI
;
Zhongrong LIU
- Publication Type:Journal Article
- Keywords:
Adenoviruses, human;
Genes;
CD40Ig
- From:
Chinese Journal of General Surgery
1994;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To construct recombinant adenovirus vector containing mouse CD40Ig fusion gene for the study of induction of donor-specific tolerance. Methods CD40Ig fusion gene was constructed by PCR overlapping technique, and was cloned into the shuttle plasmid pAdTtrack-CMV. The linearized shuttle plasmid was co-transfected into the E.coli strain BJ5183 with bone plasmid pAdeasy1, then the recombinant adenovirus plasmid was generated. The adenovirus was packaged and amplified in Cells 293. Results The recombinant virus AdCD40Ig was successfully constructed and its titer was 5?109 efu/ml. Conclusion AdCD40Ig can be used as an agent in experiment to induce donor-specific tolerance.
