Rapid Detection of Mutations of Neisseria gonorrhoeae gyrA Gene Using DNA Chip
- VernacularTitle:DNA芯片快速检测淋球菌gyrA基因突变
- Author:
Wenming ZHOU
;
Xuejun ZHANG
;
Huimin CAO
;
Sen YANG
;
Liangdan SUN
;
Jianlong ZHAO
- Publication Type:Journal Article
- Keywords:
Neisseria Gonorrhoeae;
Quinolones;
Point mutation;
Oligonucleotide array sequence analysis;
DNA gyrase
- From:
Chinese Journal of Dermatology
1994;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To develop a new method-DNA chip to be used for rapid detection of mutations of Neisseria gonorrhoeae gyrA gene. Methods Probes were designed according to the sequence of Neisseria gonorrhoeae gyrA genes, and DNA chip was fabricated accordingly. DNA fragment which contains gyrA gene mutation was amplified using PCR technique, labeled with Cy5 fluorescence, and then hybridized with DNA chip. Results of DNA sequencing were used as the control. Results All of the 50 urogenital swab specimens were detected using DNA chip. The consistency between the DNA chip and drug sensitivity test, and between the DNA chip and DNA sequencing were 100% and 98%, respectively. Conclusions DNA chip is a rapid technique with high sensitivity and specificity for the detection of mutations of Neisseria gonorrhoeae gyrA gene. This method can be used for the detection of drug resistance in clinical practice.
