Morphological Evidences of Sympathetic Nerve and Sensory Nerve Regulating Cochlear Vessels
- VernacularTitle:耳蜗血管交感及感觉神经双重调节的形态学证据
- Author:
Jianhua QIU
;
Li QIAO
;
Shunli LIN
- Publication Type:Journal Article
- Keywords:
Spiral modiolar artery;
Tyrosine hydroxylase;
Calcitonin gene-related peptide
- From:
Journal of Audiology and Speech Pathology
1998;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate localization and co-existence of the tyrosine hydroxylase (TH)and calcitonin gene-related peptide(CGRP)on the cochlear spiral modiolar artery(SMA) and its branches.Methods The SMA was dissected from the modiolus so that the entire length of the vessel and many of its branches could be observed. Immunohistochemical ABC method and confocal immunoflurescence technique were employed to localize each compound and to determine whether the TH and CGRP coexist in neurons of the SMA.Results Microscopic examination of whole vessel preparation revealed numerous TH and CGRP-positive neural networks innervating the SMA and its branches.The labeled nerve fibers showed distinct arborization ,varicosities and overlap,and were of different diameters. The overall distribution of neuronal labeling suggested a gradual decrease of innervation density from proximal (cochlear base)to more distal vessels, i.e., apical cochlear vessels and lateral radiating arterioles. Confocal immunofluorescence microscopy of double-labeled TH and CGRP neurons showed that a number of the TH-and CGRP-positive neurons were co-labeled. Thus, TH and CGRP partially co-exist within the neuronal innervation of SMA.Conclusion These findings support a hypothesis that specific neuropeptide and adrenergic neurons regulate cochlear blood flow.
