Significance of Expression of Caspase 3 and Bcl - 2 in As2O3- induced Apoptosis of Namalwa Cell Line
- VernacularTitle:半胱氨酸酶3和Bcl-2在三氧化二砷诱导Namalwa细胞凋亡过程中的表达及意义
- Author:
Enjuan ZHANG
;
Mao XING
;
Tonghua LIU
;
Linqing HUANG
- Publication Type:Journal Article
- Keywords:
arsenic trioxide;
apoptosis;
Caspase 3;
Bcl-2
- From:
China Pharmacy
1991;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE: To investigate the possible role of Caspase 3 and Bcl - 2 in arsenic trioxide(As2 O3) - induced apoptosis of Namalwa cell line .METHODS: Cell apoptosis was assessed through agarose gel elecrophroresis and the double staining of Annexin V and propidium iodide(PI), of which fluorescent intensities were measured on flow cytometer.Namalwa cells were treated with As2 O3 and DEVD - CHO, a selective inhibitor of Caspase 3,and the changes of expression of Caspase 3 and Bcl - 2 were observed .RESULTS: As2 O3 up - regulated Caspase 3 protein and down - regulated Bcl - 2 protein. DEVD-CHO inhibited the apoptosis induced by As2O3. but it had no effect on down - regulation of Bcl - 2 protein induced by As2O3.CONCLUSION:The activation of Caspase 3 may play a role in mediating apoptosis induced by As2O3 in Namalwa cells.
