Simultaneous Determination of Metronidazole and Chloramphenicol in Comedo Cream by HPLC
- VernacularTitle:高效液相色谱法测定面刺霜中甲硝唑、氯霉素的含量
- Author:
Dong LUO
;
Zhangyang WANG
;
Qing DAI
;
Guangbing RAN
- Publication Type:Journal Article
- Keywords:
HPLC;
metronidazole;
chloramphenicol;
content determination
- From:
China Pharmacy
1991;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE: To establish a HPLC method for simultaneous determination of metronidazole and chloram. phcnicol in comedo cream. METHODS: The method included a Diamonsil C18 column (250mm ? 4.6mm, 5?m) and methanol water(50 : 50,v/v)as the mobile phase and an ultraviolet detecting wavelength of 280nm .RESULTS:The calibralion curve was linear in a concentration range of ( 10 - 250)?g/ml of both metronidazole and chloramphenicol (r = 0.9 998) .The average recoveries of metronidazole and chloramphenicol were 100.53% , 100.53% and RSD were 0.79% , 0.56% respectively .CONCLUSION:The method for the determination of metronidazole and chloramphenicol was rapid and convenient. It can be used for the quality control of metronidazole and chloramphenicol in cream.
