Effect of overexpression of p16 on anion exchange function of anion exchanger 1 at HeLa cell lines
- VernacularTitle:p16蛋白过表达对哺乳动物细胞带3蛋白阴离子交换功能的影响
- Author:
Lifeng TIAN
;
Yuhui XI
;
Shuzhi BAI
;
Tianying WANG
;
Xiaoshu JIANG
;
Zhijiu ZHONG
;
Guohui FU
- Publication Type:Journal Article
- Keywords:
Protein p16;
Anion exchange protein 1, erythrocyte;
Hela cells
- From:
Chinese Journal of Pathophysiology
1986;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To study the effect of the overexpression of p16 on an anion exchange function of band 3 in HeLa cells. METHODS: The expression of p16 and band 3 in HeLa cells was detected by immunohistochemistry (IHC). The p16 cDNA was subcloned to plasmids pEGFP-C1 by PCR and identified by restriction enzyme digestion and sequencing, and then, the recombinant pEGFP-C1-p16 plasmids were transiently transfected into HeLa cells. The expression of fusion protein in HeLa cells was detected by fluorescence microscope. 6-methoxy-N-(3-sulfopropyl)-quinolinium(SPQ)fluorescent probes were used to detect the anion exchange function of band 3. RESULTS: P16 and band 3 were expressed in HeLa cells. The amplificated p16 cDNA sequence was the same as the report sequence. The transfective efficacy of pEGFP-C1-p16 was above 60%. The anion exchange function increased after the transfection of pEGFP-C1-p16 plasmids. CONCLUSION: p16 facilitates the anion exchange function of band 3 in HeLa cells.
