Influence of caveolin-1 on the ET-1-induced VSMC proliferation
- VernacularTitle:Caveolin-1在内皮素-1诱导血管平滑肌细胞增殖中的作用
- Author:
Zhi TAN
;
Jianwen CHEN
;
Jingyun PAN
;
Tinghuai WANG
- Publication Type:Journal Article
- Keywords:
Caveolins;
Caveolae;
Endothelin-1;
Muscle, smooth, vascular
- From:
Chinese Journal of Pathophysiology
1986;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the effect of caveolin-1 on the endothelin-1 (ET-1)-induced vascular smooth muscle cells (VSMC) proliferation. METHODS: The -thymidine (TdR) incorporation, immunofluorescence assays and western blotting were used in this study. RESULTS: The ETA receptor specific antagonist BQ123 inhibited the increase in TdR incorporation in response to ET-1 on VSMC. Immunofluorescence assays showed that caveolin-1 was mostly distributed in plasmalemma of VSMC. After 24 h treatment of VSMC with ET-1, the expression of caveolin-1 in VSMC was significantly decreased. Western blotting showed that ET-1 inhibited the expression of caveolin-1 in VSMC, BQ123 reversed the effect of ET-1. CONCLUSION: Caveolin-1 was mostly distributed in plasmalemma of VSMC. ET-1 downregulated caveolin-1 expression in VSMC via ETA receptor.
