Effect of adrenomedullin on L-type calcium currents and its signaling transduction process in guinea-pig ventricular myocytes
- VernacularTitle:肾上腺髓质素对豚鼠心室肌细胞L-型钙通道的作用及其信号转导机制
- Author:
Qinmei KE
;
Yimei DU
;
Yibai FENG
;
Ming TANG
;
Jiufang DI
- Publication Type:Journal Article
- Keywords:
Adrenomedullin;
Calcium channels, L-type;
Myocardium;
Signal transduction
- From:
Chinese Journal of Pathophysiology
1986;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To study the effect of adrenomedullin (ADM) on L-type calcium currents (I Ca,L) and its signaling transduction process in guinea-pig ventricular myocytes. METHODS: By using whole-cell patch clamp technique, the effect of ADM(1-100 nmol?L -1) on I Ca,Lmyocyte. Furthermore, the possible signaling transduction process of ADM on I Ca,L was investigated by observing the effects of ADM 22-52 (a specific ADM-receptor antagonist,100 nmol?L -1)+ADM(100 nmol?L -1), H-89 (a specific protein kinase A inhibitor, 10 ?mol?L -1) + ADM(100 nmol?L -1), PKC 19-36 (a specific protein kinase C inhibitor, 10 ?mol?L -1) + ADM(100 nmol?L -1) and PMA (a specific protein kinase C activator, 1 ?mol?L -1) on I Ca,L, respectively. RESULTS: ADM at the concentrations of 1-100 nmol?L -1 decreased I Ca,L in a dose-dependent manner (P
