Variation of brain derived neurotrophic factor and its mRNA expression in infancy rats after experimental bacterial meningitis
- VernacularTitle:幼年大鼠细菌性脑膜炎脑源性神经营养因子及其mRNA表达的变化
- Author:
Ling LI
;
Quanxiang SHUI
;
Xilin YU
;
Jianping LI
- Publication Type:Journal Article
- Keywords:
Meningitis,bacterial;
Brain-derived neurotrophic factor;
Gene expression;
Rats
- From:
Chinese Journal of Pathophysiology
1986;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the expression and effect of brain derived neurotrophic factor (BDNF) mRNA and its protein in infancy rats after exposure to bacterial meningitis. METHODS: Three week old rats were used to construct the models of bacterial meningitis (n=30) and mormal(n=18). At 24 h, 48 h, 5 d after inoculating, the expression of BDNF mRNA and its protein were detected by in situ hybridization and immunohistochemical staining methods, respectively. RESULTS: The increase in BDNF mRNA expression was detected by in situ hybridization at 24 h in experiment( 0.13320? 0.02750) compared to control( 0.06269? 0.01147)(P0.05). Meanwhile, in the brain from the experiment rats, strong positive hybridization and immunoreactivity were observed in the infiltrated inflammatory cells in leptomeninges, subarachnoid cavity, ventricles and brain parenchyma. CONCLUSIONS: These results support the hypothesis that BDNF might play a neuroprotective role in brain damage process in bacterial meningitis attacked rats. BDNF might take part in imune response. These results also support the hypothesis that BDNF has some relationships with other inflammatory mediators during acute inflammatory response of bacterial meningitis.
