Expression and functional role of p38MAPK in the kidney after unilateral ureteral obstruction in rats
- VernacularTitle:p38MAPK在大鼠梗阻性肾病肾组织中的表达及可能作用
- Author:
Shenglang ZHU
;
Xueqing YU
;
Ning LOU
;
Xunhua ZHENG
;
Yongquan LI
- Publication Type:Journal Article
- Keywords:
Ureteral obstruction;
p38MAP kinase;
Transforming growth factor beta;
Renal fibrosis
- From:
Chinese Journal of Pathophysiology
1986;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the expression and functional role of p38MAPK in the kidney after unilateral ureteral obstruction in rats. METHODS: Unilateral ureteral obstruction (UUO) models were induced by ligating the left ureter. Rats were sacrificed at 1 h, 3 h, 6 h, 12 h, 1, 3, 5, 7, 14, 21, and 28 days after UUO was initiated. p38MAPK activity was assayed by immunohistochemical staining and specific substrate phosphorylation with immunoprecipitation and Western blotting. TGF? mRNA and protein expression were analyzed with in situ hybridization and immunohistochemical stainning. RESULTS: A basic p38MAPK activity was detectable in the normal kidney(0.22?0.06). p38MAPK pathway was rapidly activated at 1 hour(0.45?0.14 vs control, P
